Journal: Endocrinology

Article Title: Steroidogenic Factor 1 Promotes Aggressive Growth of Castration-Resistant Prostate Cancer Cells by Stimulating Steroid Synthesis and Cell Proliferation

doi: 10.1210/en.2013-1583

Figure Lengend Snippet: SF1 mediates growth of BCaPT10 prostate cell xenografts within a steroid-depleted environment. Tissue images of shNEG (A) and shSF1 (B) cells grafted under the kidney capsule of castrated nude mice after 2 months of growth. White arrows denote tissue derived from grafts surrounded by the kidney. C, Tumor volumes from each individual sample (n = 7 shNEG, n = 4 shSF1) are represented with the mean and SD marked by error bars. *, P < .05; Student t test, two-tailed, equal variance. Hematoxylin and eosin–stained sections from shNEG-derived (D) and shSF1-derived (E) tumors (K, kidney; G, graft, dotted line separates graft from kidney). F and G, High-magnification hematoxylin and eosin images of grafts from shNEG (F) and shSF1 (G) cells showing cell architecture corresponding to neighboring serial sections used for immunohistochemical analysis with anti-SV40 T Ag (brown, insets), which was used to validate their origin from the parent BCaPT10 cell line. F, White arrows mark examples of mitotic figures in dividing cells within the shNEG graft that were also positive for SV40 T Ag (white arrowheads within inset). H and I, Ki67-stained cells (brown) comparing cell proliferation between shNEG (H) and shSF1 (I) grafts; insets indicate serial sections stained with SV40 T Ag with white arrows highlighting examples of cells stained for both Ki67 and SV40 T Ag. J and K, Immunohistochemical analysis was performed to identify SF1 positive cells (brown) in shNEG (J) and shSF1 (K) grafts; insets show serial sections stained for 3βHSD. White arrows indicate examples of cells positive for both SF1 and 3βHSD. Scale bars correspond to 0.5 mm (D and E), 0.05 mm (F, G and F and G insets), 0.1 mm (H, I, J, and K), and .01 mm (H, I, J, and K insets). 3,3′-DAB staining was counterstained with hematoxylin (blue, F and G insets and H, I, J, and K).

Article Snippet: For immunohistochemical analysis, sections were dewaxed and rehydrated, and the Vector Mouse on Mouse (M.O.M.) (Vector Laboratories) or rabbit IgG kits were used as indicated by the manufacturers with anti-SV40 T Ag (Santa Cruz Biotechnology), Ki67 (Abcam), SF1 (clone N1665; Invitrogen), 3-β-hydroxysteroid dehydrogenase (3-βHSD) (kindly provided by Ken-Ichirou Morohashi, Kyushu University, Fukuoka, Japan), and a 3,3′-diaminobenzidine (DAB) peroxidase substrate kit (Vector Laboratories).

Techniques: Derivative Assay, Two Tailed Test, Staining, Immunohistochemical staining

Journal: Science advances

Article Title: Mitochondrial-targeted antioxidant attenuates preeclampsia-like phenotypes induced by syncytiotrophoblast-specific Gαq signaling.

doi: 10.1126/sciadv.adg8118

Figure Lengend Snippet: Fig. 2. Exogenous fetoplacental Gαq activation leads to severe pregnancy impairments in mice (hM3DqF/F dam x Actb-Cre+/+ sire, GD 14.5). (A) Twenty-four–hour maternal urine protein excretion. (B) Maternal plasma sFLT1. (C) Placental VEGF protein levels. (D) Hematoxylin and eosin stain of glomeruli. Scale bars, 25 μm. (E) Rep- resentative labyrinth image of diaminobenzidine (DAB) immunostained for CD31. Scale bars, 50 μm. (F) Percentage area of CD31-positive placenta. (G) Hematoxylin and eosin stain of fetoplacental unit. Scale bars, 500 μm (left), 50 μm (middle), and 10 μm (right). Each datapoint represents a biological replicate. *P < 0.05, independent samples t test (two-tailed).

Article Snippet: Horseradish peroxidase SignalStain Boost Detection Reagent for rabbit immunoglobulin G (Cell Signaling, 8114) and diaminobenzidine (DAB) substrate (Cell Signaling, catalog no. 8059) were used for CD31 staining.

Techniques: Activation Assay, Clinical Proteomics, H&E Stain, Two Tailed Test